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the t7 expression vectors pet 29-b  (Millipore)


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    Structured Review

    Millipore the t7 expression vectors pet 29-b
    The T7 Expression Vectors Pet 29 B, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+29-b/pet+29+b/us11796534-450-42-47
    Average 90 stars, based on 1 article reviews
    the t7 expression vectors pet 29-b - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: Development of recombinant Aleuria aurantia lectins with altered binding specificities to fucosylated glycans
    Article Snippet: The synthesized gene product was subsequently subcloned as an NdeI-XhoI fragment from the pUC57 cloning vector into the T7 expression vectors pET 29-b (Novagen) and pQE-T7 (Qiagen) which results in the addition of the sequence for an in frame histidine tag at the C-terminal end of the AAL gene sequence.

    Article Title: Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarker of hepatocellular carcinoma
    Article Snippet: HPLC chromatogram were acquired on Waters Alliance HPLC system complemented with Waters fluorescence detector and Millenium Chromatography Manager software for quantification (Milford, MA, USA).

    Article Title: Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarker of hepatocellular carcinoma
    Article Snippet: All other reagents and chemicals were purchased and used from Sigma (St. Louis, MO, USA) as received without further purification.

    Recombinant:

    Article Title: Development of recombinant Aleuria aurantia lectins with altered binding specificities to fucosylated glycans
    Article Snippet: The synthesized gene product was subsequently subcloned as an NdeI-XhoI fragment from the pUC57 cloning vector into the T7 expression vectors pET 29-b (Novagen) and pQE-T7 (Qiagen) which results in the addition of the sequence for an in frame histidine tag at the C-terminal end of the AAL gene sequence.

    Article Title: Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarker of hepatocellular carcinoma
    Article Snippet: HPLC chromatogram were acquired on Waters Alliance HPLC system complemented with Waters fluorescence detector and Millenium Chromatography Manager software for quantification (Milford, MA, USA).

    Article Title: Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarker of hepatocellular carcinoma
    Article Snippet: All other reagents and chemicals were purchased and used from Sigma (St. Louis, MO, USA) as received without further purification.

    Clone Assay:

    Article Title: Development of recombinant Aleuria aurantia lectins with altered binding specificities to fucosylated glycans
    Article Snippet: The synthesized gene product was subsequently subcloned as an NdeI-XhoI fragment from the pUC57 cloning vector into the T7 expression vectors pET 29-b (Novagen) and pQE-T7 (Qiagen) which results in the addition of the sequence for an in frame histidine tag at the C-terminal end of the AAL gene sequence.

    Article Title: Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarker of hepatocellular carcinoma
    Article Snippet: HPLC chromatogram were acquired on Waters Alliance HPLC system complemented with Waters fluorescence detector and Millenium Chromatography Manager software for quantification (Milford, MA, USA).

    Article Title: Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarker of hepatocellular carcinoma
    Article Snippet: All other reagents and chemicals were purchased and used from Sigma (St. Louis, MO, USA) as received without further purification.

    Plasmid Preparation:

    Article Title: Development of recombinant Aleuria aurantia lectins with altered binding specificities to fucosylated glycans
    Article Snippet: The synthesized gene product was subsequently subcloned as an NdeI-XhoI fragment from the pUC57 cloning vector into the T7 expression vectors pET 29-b (Novagen) and pQE-T7 (Qiagen) which results in the addition of the sequence for an in frame histidine tag at the C-terminal end of the AAL gene sequence.

    Article Title: Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarker of hepatocellular carcinoma
    Article Snippet: HPLC chromatogram were acquired on Waters Alliance HPLC system complemented with Waters fluorescence detector and Millenium Chromatography Manager software for quantification (Milford, MA, USA).

    Article Title: Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarker of hepatocellular carcinoma
    Article Snippet: All other reagents and chemicals were purchased and used from Sigma (St. Louis, MO, USA) as received without further purification.

    Expressing:

    Article Title: Development of recombinant Aleuria aurantia lectins with altered binding specificities to fucosylated glycans
    Article Snippet: The synthesized gene product was subsequently subcloned as an NdeI-XhoI fragment from the pUC57 cloning vector into the T7 expression vectors pET 29-b (Novagen) and pQE-T7 (Qiagen) which results in the addition of the sequence for an in frame histidine tag at the C-terminal end of the AAL gene sequence.

    Article Title: Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarker of hepatocellular carcinoma
    Article Snippet: HPLC chromatogram were acquired on Waters Alliance HPLC system complemented with Waters fluorescence detector and Millenium Chromatography Manager software for quantification (Milford, MA, USA).

    Article Title: Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarker of hepatocellular carcinoma
    Article Snippet: All other reagents and chemicals were purchased and used from Sigma (St. Louis, MO, USA) as received without further purification.

    Sequencing:

    Article Title: Development of recombinant Aleuria aurantia lectins with altered binding specificities to fucosylated glycans
    Article Snippet: The synthesized gene product was subsequently subcloned as an NdeI-XhoI fragment from the pUC57 cloning vector into the T7 expression vectors pET 29-b (Novagen) and pQE-T7 (Qiagen) which results in the addition of the sequence for an in frame histidine tag at the C-terminal end of the AAL gene sequence.

    Article Title: Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarker of hepatocellular carcinoma
    Article Snippet: HPLC chromatogram were acquired on Waters Alliance HPLC system complemented with Waters fluorescence detector and Millenium Chromatography Manager software for quantification (Milford, MA, USA).

    Article Title: Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarker of hepatocellular carcinoma
    Article Snippet: All other reagents and chemicals were purchased and used from Sigma (St. Louis, MO, USA) as received without further purification.

    Mutagenesis:

    Article Title: Development of recombinant Aleuria aurantia lectins with altered binding specificities to fucosylated glycans
    Article Snippet: The synthesized gene product was subsequently subcloned as an NdeI-XhoI fragment from the pUC57 cloning vector into the T7 expression vectors pET 29-b (Novagen) and pQE-T7 (Qiagen) which results in the addition of the sequence for an in frame histidine tag at the C-terminal end of the AAL gene sequence.

    Article Title: Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarker of hepatocellular carcinoma
    Article Snippet: HPLC chromatogram were acquired on Waters Alliance HPLC system complemented with Waters fluorescence detector and Millenium Chromatography Manager software for quantification (Milford, MA, USA).

    Article Title: Development and application of a novel recombinant Aleuria aurantia lectin with enhanced core fucose binding for identification of glycoprotein biomarker of hepatocellular carcinoma
    Article Snippet: All other reagents and chemicals were purchased and used from Sigma (St. Louis, MO, USA) as received without further purification.



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